Partial domain adaptation enables cross domain cell type annotation between scRNA-seq and snRNA-seq

Authors: Xiran Chen, Quan Zou, Qinyu Cai, Xiaofeng Chen, Weikai Li et al.

Year: 2025

q-bio.GN

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2025
Published
6
Authors

Abstract

Accurate cell type annotation across datasets is a key challenge in single-cell analysis. snRNA-seq enables profiling of frozen or difficult-to-dissociate tissues, complementing scRNA-seq by capturing fragile or rare cell types. However, cross-annotation between these two datasets remains largely unexplored, as existing methods treat them independently. We introduce ScNucAdapt, a method designed for cross-annotation between paired and unpaired scRNA-seq and snRNA-seq datasets. To address distributional and cell composition differences, ScNucAdapt employs partial domain adaptation. Experiments across both unpaired and paired scRNA-seq and snRNA-seq show that ScNucAdapt achieves robust and accurate cell type annotation, outperforming existing approaches. Therefore, ScNucAdapt provides a practical framework for the cross-domain cell type annotation between scRNA-seq and snRNA seq data.

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